Hanna Instruments HI 3857 - Manual

Instruction Manual
www.hannainst.com
Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instructions carefully before using the
chemical test kit. It will provide you with the necessary
information for correct use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
• HI 3857A-0 Detergents Reagent, 1 bottle with drop-
per (15 mL);
• HI 3857B-0 Detergents Reagent, 1 bottle with drop-
per (15 mL);
• Chloroform, 1 glass bottle (180 mL);
• Demineralizer Bottle, 1 bottle with filter cap for
about 12 liters of deionized water (depending on the
hardness level of water to be treated).;
• 1 checker disc;
• 2 long glass vials (30 mL) with caps;
• 1 long plastic pipette;
• 1 plastic pipette (3 mL);
• 1 plastic pipette (1 mL).
Note: Any damaged or defective item must be returned in
its original packing materials.
HI 3857
Detergents
Test Kit
ISTR3857 11/99 PRINTED IN ITALY
Detergents can enter water and wastewater by discharge of
domestic and industrial cleansing waters. The most widely
used detergents are linear alkyl sulfonates (LAS) and alkyl
benzene sulfonates (ABS): LAS are preferable to ABS be-
cause they are biodegradable, thus readily decomposed by
microorganisms. The presence of anionic LAS/ABS detergents
in natural waters should be below 0.1 mg/L and in raw
domestic wastewater in the range from 1 to 20 mg/L.
Note: mg/L is equivalent to ppm (parts per million).
Range 0.00 to 1.30 mg/L as ABS/LAS
Smallest Increment 0.02 mg/L
Analysis Method Colorimetric
Sample Size 20 mL
Number of Tests 35
Case Dimensions 235x175x115 mm (9.2x6.9x4.5")
150x150x220 mm (5.9x5.9x8.7")
Shipping Weight 1245 g (43.9 oz.)
SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS
SIGNIFICANCE AND USE SIGNIFICANCE AND USE SIGNIFICANCE AND USE SIGNIFICANCE AND USE SIGNIFICANCE AND USE
CHEMICAL REACTION CHEMICAL REACTION CHEMICAL REACTION CHEMICAL REACTION CHEMICAL REACTION
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
Note: Non soap anionic surfactants commonly used in
detergent formulations are strongly responsive. Soaps,
as alkali salts of fatty acids do not respond to the
test.
1- Remove the cap and
fill the Demineralizer
Bottle with tap water.
2- Replace the cap and
shake gently for at least
2 minutes. The deminer-
alized water is now ready.
Note: The ion exchange resin contained in the Demineral-
izer Bottle is provided with an indicator substance.
The indicator will change from green to blue when
the resin has been exhausted and has to be re-
placed with a new one.
3- Fill one glass vial with 5 mL of sample.
This is the blank. Insert the blank into
the left hand opening of the checker disc.
4- Fill the other glass vial with 20 mL of
the sample, up to the mark.
5- Add 2 drops of HI
3857A-0 Detergents
Reagent. Replace the
cap and swirl to mix.
INSTRUCTIONS INSTRUCTIONS INSTRUCTIONS INSTRUCTIONS INSTRUCTIONS
The aqueous solution containing the detergents is treated
with the colored indicator. The reaction product can be
extracted in the chloroform layer, whilst the original dyestuff
is insoluble in the organic medium. The intensity of the color
developed is proportional to the concentration of the deter-
gents present.
6- Add 3 drops of
HI 3857B-0 De-
t e r g e n t s
Reagent, re-
place the cap
and swirl to mix.
The solution will
turn a light blue color.
7- Using the 3 mL plastic pipette,
add exactly 5 mL of Chloroform.
The chloroform will sink to the
bottom of the vial because it is
heavier than water.
8- Cap the vial tight and mix by inverting it slowly for 30
seconds. Let it stand for about 1 minute until the
chloroform layer separates from the aqueous layer. The
color of the aqueous layer will fade slightly, while the
chloroform layer turns slightly blue.
9- Using the long plastic pipette, remove the upper
aqueous layer and dispose of it. Do not remove
the lower chloroform layer too!
10-Open the upper part of the Dem-
ineralizer Bottle cap and, squirting
gently, add to the vial 20 mL of
demineralized water, up to the 25
mL mark.
11- Add 2 drops of HI 3857A-0
reagent.
12- Cap the vial tight and mix by inverting slowly for 30
seconds. Let it stand for at least 1 minute - until the
aqueous layer is unclouded- to allow the chloroform
5 mL
20 mL
5 mL