Hoefer SE400 - Manual (Page 31)

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p23
2.6 After electrophoresis
1
Once the tracking dye reaches the bottom of the gel,
turn off the power supply and disconnect the leads.
Remove the safety lid, using finger leverage — rest
your thumbs on the top of the cams and gently pull
the lid up with your index fingers. Once loose, lift the
lid straight up and then out to clear the ledge on the
upper buffer chamber.
2
Pour out the buffer by inverting the unit over a sink.
Release the upper buffer chamber by removing the
cams. Lift the chamber off and lift the sandwich out
of the lower chamber.
3
Unscrew the clamps from the sandwiches and remove.
Gently loosen and then slide away both spacers. Use
the Wonder Wedge plate separator tool to separate
the plates.
4
Carefully lift off one glass plate. Handle the gel with
care to avoid damaging it. Over an empty stain tray,
either invert the plate holding the gel near the bottom
of the tray and lift one corner so that the gel drops
into the tray, or, if the gel is thick enough to handle,
lift it and place into the tray. Add enough fixative or
stain to completely submerge the gel.
5
Clean the unit as described in “Care and
maintenance” on page 24.
Tip: To avoid splashing, add
staining or fixative solution
to the tray after the gel is
transferred.
Note: Use only flexible plastic
prying tools to avoid chipping
the glass plates.