Hoefer SE615 - Manual (Page 6)

Casting with divider plates
You may find it useful to double the number of
gels run simultaneously in one electrophoresis
unit. This can be accomplished by casting a
“club sandwich.” A club sandwich contains a
notched glass divider plate and a second pair
of spacers between the standard plates, and
thus forms two layers of gel between three glass
plates (see Fig 3 on page 8.) Divider plates are
available for the SE400 and SE600.
The maximum number of gels you can cast
simultaneously, using divider plates (two gels
per sandwich), is shown in Table 2.
Table 2. Maximum number of gels
cast with divider plates
0.75-mm 1.0-mm 1.5-mm
spacers spacers spacers
SE615 14 14 12
SE675 6 6 4
See Fig 1 on page 4 to identify the components
of the multiple gel casters. For information on
parts, accessories and related equipment, see
page 22.
Pouring second-dimension slab gels
When preparing second-dimension slab gels in
the multiple casters, it is important to pour the
slabs to the correct height.
If you are using IPG strips, you do not need a
stacking gel. Pour the second-dimension gel to
0.5–1.0 cm below the top of the sandwich for
standard casting, or 1 cm below the notch if
you are casting with divider plates. This allows
ample room to hold the IPG strips between the
two glass plates. Use 1.0- or 1.5-mm spacers
with IPG strips.
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