Hoefer PR648 - Manual (Page 9)

Troubleshooting
problem solution
If the sample area on the membrane is
larger than the slot size, or if the solution
is leaking out of the slot…
Screws are tightened too Carefully follow the tightening instructions in Steps 6 and 7
far or not tightened far enough. in Setting up the Hoefer PR648 slot blot.
The membrane is not positioned Make sure the membrane is flat and fits completely within
correctly with the recess. the recess with its edges parallel to the recess edges.
The membrane is dry. Before blotting, be sure to soak the membrane in buffer to
wet it thoroughly.
If small impressions show on the
membrane around the blot…
This is normal. The impressions, which indicate a tight seal, will not affect
your results if you scan the membrane in reflectance mode.
Place the membrane flat on the smooth side of a porous
polyethylene sheet, and dry it for several minutes in a gel dryer
without heat.
If there are bubbles in the slot…
There are proteins or detergent in the To prevent bubbles from forming, be sure to pipet the sample
sample. Or, the sample was squirted slowly and carefully against the bottom side-wall of the slot.
rapidly into the slot. If necessary, flush bubbles out with a pipette tip.
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