GE GM-1 - Manual (Page 2)

i 56-2853-00 AE, 2002-12 • p2
Dismantling the Gradient Mixer
The reservoir cylinders (7), control valve (12) and flow
control valve (18) may be removed from the base plate by
unscrewing them. To remove the stirrer blade (6) from the
motor unit (1, 2), unscrew the nylon screw (5) a few turns.
It is not necessary to remove the screw completely. The lid of
the motor unit should not be removed.
6. Operation
Operating the Gradient Mixer
1. Close the control valve (12) and flow control valve (18)
and pour the required amount of starting solution into
the mixing reservoir. The graduations may be used as an
approximate guide to the volume of liquid in the
reservoirs.
2. Mount the motor unit (1, 2), with the stirrer blade (6),
on the mixing reservoir.
3. Open the control valve (12) slightly so that the channel
between the reservoirs fills with solution and a small
amount of liquid emerges into the other reservoir. Close
the control valve.
4. Pour the gradient-forming solution into the empty
reservoir until the liquid levels in the two reservoirs are
nearly the same. The level in the mixing reservoir should
be slightly higher than the level in the other reservoir to
avoid a step at the beginning of the elution.
5. Open the control valve (12) fully, one complete
revolution, start the motor and begin the elution by
opening the flow control valve (18). If a pump is used to
control the eluent flow, the flow control valve (18)
should be fully open, one complete revolution .
The Gradient Mixer will give linear ionic strength gradients
and reproducible, but non-linear, pH gradients. The exact
shape of the pH gradient obtained will depend on the buffer
salts used and the ionic strengths and pH’s of the buffer
solutions. The use of gradient elution in ion exchange
chromatography is described in the booklet “Ion Exchange
Chromatography – principles and methods.”
7. Maintenance
Warning!
When using hazardous chemicals, all suitable protective
measures such as protective glasses, must be taken.
Cleaning
Ordinary laboratory detergents may be used to clean the
gradient mixer. Enzyme detergents are recommended for
removing protein aceous contaminants. The mixer, including
the stirrer blade but not the motor unit, may be autoclaved
at 121 °C for 20 min.
Caution!
Do not autoclave the motor unit or drain the motor in
strong detergents.
8. Technical specification
Mixing chamber 300 ml
volume
Environment +4–40 °C
20–95% relative humidity
Material of Polymethylpentene,
wetter parts Polypropylene, glass fibre reinforced
polypropylene and fluororubber.
Chemical resistans The wetted parts are resistant to
aqueous and organic solvents
commonly used in ion change
chromatography and solutions
containing inorganic and organic
acids, alkalis and alcohols.
Power requirements 100–120 VAC or 220–240 VAC,
50–60 Hz
Power consumption 7 VA
Safety standards This products meets the requirements
of the Low Voltage Directive (LVD)
73/23/EEC through the harmonized
standards EN61010-1 and EN60742.
Note: The declaration of conformity is valid for the
instrument when it is:
• used in laboratory locations,
• used in the same state as it was delivered from
GE Healthcare Bio-Sciences AB
except for alterations described in the
user manual,
• used as a “stand alone” unit or connected to other CE
labelled GE Healthcare Bio-Sciences AB instruments or other
products as recommended.