Olympus IX2 RFAEVA2 - Manual (Page 14)

11
TOTAL INTERNAL REFLECTION FLUORESCENCE
OBSERVATION
Because this observation method employs laser beam, be sure to observe the
following precautions.
3-1 Conditions of Observation
Be sure to observe the following laser operating environment conditions.
· Temperature: 18 to 28°C (performance guaranteed), 10 to 35°C (laser oscillation guaranteed).
· Humidity: 30% to 80% (without condensation)
· Excitation wavelengths: 400 to 750 nm with both laser and mercury burner.
Observe the following precautions in the total internal reflection fluorescence observation.
· Applicable objectives for total internal reflection fluorescence observation
Cell specimens and fluorescent molecules: Apo100XOHR (NA 1.65), PlanApoN60XO TIRFM (made to order, NA 1.45).
Fluorescent molecules: UPlanSApo100XO (NA 1.40), PlanApoN60XO (NA 1.42).
· A special cover glass and immersion oil are required when using the Apo100XOHR.
· The total internal reflection fluorescence mirror unit can be used by removing the excitation filter of the excita-
tion mirror unit.
· Never remove a part on which a warning panel is attached.
· Be sure to set the interlock key switch to OFF and make sure that the laser emission indicator
is OFF before proceeding to the objective switching or fluorescence turret switching.
Excitation Mirror Unit Laser (Wavelength)
IB excitation U-M)IB3
Ar laser (488 nm)
U-M)IBA3
G excitation U-M)G2 HeNe-G laser (543 nm)
U-MWIG3 Nd-YAG laser (532 nm)
U-M)IGA3
R excitation (Commercially HeNe-R laser (633 nm)
available product)
IY excitation U-MWIY2 Kr laser (568 nm)
BV excitation U-M)BV2 He-Cd laser (442 nm)
· The character(s) in ) can be SW, W or N. Type 2 excitation mirror units can also be used.
· For the multi-excitation mirror units, consult Olympus.
· For IB excitation fluorescence observation using the mercury burner, it is required to prepare a separate IB
excitation mirror unit incorporating an excitation filter, apart from the total internal reflection fluorescence mirror
unit.
· Total internal reflection observation is not available with the binocular tube light path (because the laser will not
be emitted unless the binocular tube shutter is engaged in the light path).
· The observation field number is 11.7 with both laser and mercury burner.