Ocean Optics LPC-500CM - Manual (Page 20)

3: Operation
12 041-00000-010-02-201106
5. Flush the LWCC with de-ionized water or experimental buffer solution using a pump or a syringe
at a rate of 1mL/minute and observe the light intensity or absorbance baseline on the detector.
Continue flushing until the signal is stable. (See
Troubleshooting in Chapter 4: Maintenance if
the signal does not stabilize appropriately.)
Measuring Discrete Samples
Discrete samples can be measured with the LWCC by introducing the sample with a syringe. Sample
volumes approximately 1.5 – 3 times the cell volume are necessary to fill the LWCC. When injecting a
sample or reference solution, apply consistent hand pressure to the syringe. Small variations in baseline
levels may result from using this method.
► Procedure
1. Using fiber optic cables, connect the LWCC to a light source and a detector.
2. Clean the LWCC using the standard cleaning procedure described in Chapter 4: Maintenance .
3. Flush the LWCC with de-ionized water or experimental buffer solution using a syringe and
observe the light intensity or absorbance baseline on the detector until the signal is stable.
4. Introduce the sample slowly into the LWCC with a syringe using steady pressure to avoid
generating air bubbles.