Carolina 3M Petrifilm Coliform Count Plates - Manual (Page 5)

5
4. Roll the top film onto the bottom film. Do not drop the top film down
(fig. 4).
Figure 4
5. With the flat side down (not the side with the circular ridge), place the
spreader on the top film over the inoculum (fig. 5).
Figure 5
6. Gently apply pressure on the spreader to distribute the inoculum over
a circular area. Do not twist or slide the spreader (fig. 6).
Figure 6
Ridge