Carolina Bottled Media - Manual (Page 5)

5
or a thoroughly flamed wire loop.
Note: You must use a new,
STERILE
swab or a flame-sterilized loop for
each plate.
3. Rub the swab or loop over or place it in the material to be cultured.
4. Lift the lid of an agar plate.
5. Streak the plate. Use either a simple zigzag streak (Figure 1) or an
isolation streaking method (Figure 2). Quickly replace the lid after making
each streak. Remember: For isolation streaking, a wire loop must be
used.
Figure 1. Simple streak (use sterile loop or swab)
1 2
3 4
Figure 2. Isolation streaking (use metal loop only and flame it between streaks)