Luminex Flock Monitor Quick Guide - Manual

Quick Guide
89-30000-00-261 Revision A - Page 1 of 2
Sample, Reagent and Plate Preparation
Before setting up the Flock Monitor assay plate, use the following procedures to prepare fresh reagents and sample
dilutions. Allow all components to equilibrate to room temperature, and prepare aliquots and dilutions immediately
prior to performing the assay.
• May be a LifeSep 96F hand held magnet for maual washing (see http://www.luminexcorp.
com/support/Magnetic%20Microspheres/Washing_Method_whitepaper.pdf for more details)
• May be a Bio Tek ELx405 or Tecan Hydroflex washer equipped with a washer
manufacturer provided magnet for automated washing
MAGNETIC
SEPERATION
DEVICE
This Quick Guide is not intended to replace the xMAP® Flock Monitor (IBDV, NDV, IBV, REO) Assay Package Insert (document number 89-60000-
00-070 rev. 9). Please refer to the Package Insert for complete background, instructions, warnings and procedure precautions.
• Calculate how much conjugate you will need with the following formula:
Required SA-PE conjugate volume = (0.10 mL) x (number of wells*) + (10%)
• Aliquot this amount into a reagent trough, then cover with foil to protect from light.
• Promptly return SA-PE conjugate to 2-8°C storage.
• Calculate how much conjugate you will need with the following formula:
Required conjugate volume = (0.10 mL) x (number of wells*) + (10%)
• Aliquot this amount into a reagent trough, then cover with foil to protect from light.
• Promptly return detection antibody to 2-8°C storage.
SA-PE
CONJUGATE
DETECTION
ANTIBODY
• WASH Buffer is provided ready-to-use in final dilution. WASH
BUFFER
• Calculate the volume of microspheres you will need with the following formula:
Required microsphere volume = (0.05 mL) x (number of wells) + (10%)
• Aliquot the required microsphere volume into another tube or reagent trough, shield
from light, and set aside for use in assay setup.
• Dilute all samples 1:500 using Sample DILUENT. You will need 50 µL of each diluted
sample per well.
• IMPORTANT - Vortex samples before use and after dilution.
• Dilute CONTROLS labeled (-) and (+) 1:500 using Sample DILUENT. You will need 50 µL
of each diluted control per well.
• IMPORTANT - Vortex controls before use and after dilution.
SAMPLES
CONTROLS
MICROSPHERE
MIX
*Consult the product insert for more information
Flock Monitor
I B D V
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N D V
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I B V
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R E O