Multichannel Systems MEA1060-Up-BC Manual - Manual (Page 38)

MEA Amplifier with Blanking Circuit for Upright Microscopes
38
Single pulses versus repeated pulses
Interestingly, the behavior of MEA electrodes when applying pulses that are repeated after
some milliseconds, is much better than when a single pulse is applied. You can observe that
the kinetics improve, that is, you need a shorter Wait, following the application of a few pulses.
This is generally not a problem, as stimulation in regular intervals is the standard case for MEA
experiments, but should be considered when testing out your experimental settings.
Repeated bipolar biphasic voltage stimulation on electrodes 74 and 75.
The figure illustrates a stimulation with biphasic pulses at a 50 ms interval:
200 mV stimulus amplitude, 60 μs duration, 3D-MEA filled with PBS, silver pellet
as bath electrode. The non-stimulating electrodes show an excellent behavior
without stimulus artifacts.
Single shot bipolar biphasic voltage stimulation on electrodes 74 and 75.
The figure illustrates a stimulation with single biphasic pulses. The experimental settings
are identical to the preceding figure. The stimulus artifact performance is not so good
as with the repeated stimulation.