Eppendorf G0.5 µPlate - Manual (Page 28)

Calculations
Eppendorf
® μPlate G0.5
English (EN)
28
9.3.2 Calculations based on an individual blank value
The well-specific absorbance value at 340 nm is subtracted from the corresponding
absorbance value at 260 nm. Then, a value is determined using the corresponding value
for each well used for the sample measurement.
Ext
A1
= (Ext
260 A1
– Ext
340 A1
) – Ext
A1 individual blank value
[OD]
Ext
A2
= (Ext
260 A2
– Ext
340 A2
) – Ext
A2 individual blank value
[OD]
Ext
B1
= (Ext
260 B1
– Ext
340 B1
) – Ext
B1 individual blank value
[OD]
etc.
The absorbance values at 280 nm will also be corrected using the corresponding
absorbance values at 340 nm. The corrected absorbance values will be used to calculate
the 260/280 ratio.