7 influence of the cell density, 3 optimizing the parameters – Eppendorf Multiporator - Electroporation User Manual
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A cell density of 1 x 10
6
cells/ml is recommended for electroporation since the electrical field is still effective on the cells
in this range. When high cell densities (>3 x 10
6
) are used, homogeneous field conditions can no longer be guaranteed,
i.e. the cells are no longer evenly exposed to the electrical field. This may lead to cell fusion and cause the transfection
rate to decrease.
However, using a cell density of <1 x 10
6
cells/ml should have no negative effect on the transfection rate.
Bibliography
3) Zimmermann, U., Effect of high intensity electric field pulses on eucaryotic cell membranes.
In: Effect of high intensity electric field pulses on eucaryotic cell membranes.
U. Zimmermann and G. Neill, editors. pp. 1-106. CRC Press, Boca Raton, 1996.
3.7 Influence of the cell density
3 Optimizing the parameters
3 Optimizing the parameters
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