Eppendorf UVette - Manual (Page 6)

6
Eppendorf® UVette® — Instructions for use
4.1.1 Eppendorf BioPhotometer/Eppendorf BioSpectrometer
1. Position the cuvette so the required optical path length points toward the light path.
2. During insertion, press the cuvette all the way down against a light resistance.
4.1.2 Inserting the cuvette and adapter in photometers from other
manufacturers
Function of the adapter
•
Adjust the height of the light beam in the corresponding photometer.
•
Diaphragm function:
– Hides the light bundle on the cross-section of the UVette measuring window
– Reduction of stray light
Like measurements with commercial, quartz glass ultra-micro cuvettes, hiding the light
bundle reduces the upper limit of the photometric measuring range. Therefore, the
usable, linear measuring range may be lower than the value specified for the
photometer. To calculate the highest absorbance values that can be measured with the
adapter/UVette, determine the measuring range by measuring a dilution series of the
desired wavelength.
Additional information is available from Application Support (contact information is on
the last page of these instructions) or at: www.eppendorf.com.
1. Select the adapter that matches the light path height of the device.
2. Insert the UVette in the adapter.
3. Insert the adapter and UVette in the cuvette holder of the photometer. Firmly press
the UVette downward.
The UVette and adapter may not have any lateral play in the cuvette shaft of the
device.
4.2 Measuring blanks and samples
Hint!
Precise and reproducible positioning of the adapter and cuvette in the cuvette
holder is a decisive factor for the reproducibility of the measurement.
NOTICE!
NOTICE! Faulty measured values due to incorrect orientation of the
cuvette.
 Always insert the cuvette in the same direction within a series of
measurements. Do not rotate the cuvette 180° during a series of
measurements.
NOTICE!
NOTICE! Faulty measured values due to repeated use of the cuvette.
If the UVette is used several times, material changes can lead to incorrect
measured values.
 Each UVette may only be used for a single measuring procedure with one
blank value and one sample value.
 The cuvette blank must be determined again for each cuvette.