Labnet Biopette A Autoclavable Multichannel Pipettes - Manual (Page 5)

3 4
M U L T I C H A N N E L
If the knob is accidentally turned too far, ie is
set lower than the required value, the process
must be repeated. The approach to the set vol-
ume must always be made in the order of
decreasing value.
3 - METHOD OF PIPETTING
Fit the tips onto cones of the multichannel module.
In order to put tips on the multichannel shafts
hold the pipette vertically and press it against
the tips in the rack box, until the shafts retreat
about 1.5 mm into the multichannel module. The
suspension system of the shafts ensures even
and tight sealing of tips. The rolling movement
does not have to be performed to seal the tips
tightly. The liquid aspirated into the tips should
not flow out by gravity from properly fixed tips.
While holding the pipette in a vertical position,
tips should be immersed in the liquid to a depth
of 2-4 mm, and flushed once by drawing a dose
of liquid and dispensing it out with slow and
steady movement.
Next, still holding the pipette vertically, the ope-
rator should press the pipetting button until the
first resistance point is felt, and immerse the tips
in the liquid to the depth of 2-4 mm, (Fig. 3B).
Releasing the pipetting button with a slow and
uniform movement during 2-3 seconds, the liquid
should be drawn into the tips, (Fig. 3B) which
should then be lifted above the liquid’s surface.
The pipette should next be positioned at an
angle of 10-45 degrees in relation to inner walls
of destination vessels and tips should
be emptied by pressing the pipetting button
slowly until the first resistance point is felt,
(Fig. 3C). After waiting one second, the pipetting
button should be pressed to the second resis-
tance point in order to expel remaining liquid,
(Fig. 3D). Then tips should be lifted out from the
vessels while maintaining contact between
the ends of the tips and the inner walls of the
vessel until the pipetting button has been rela-
sed, (Fig. 3E). Finally, pressing the ejector but-
ton, the operator should separate the tips from
multichannel module’s cones, (Fig. 3F).
the procedures outlined in the EN ISO 8655
standard.
The pipette design enables the user to perform
the recalibration process according to the rules
presented in section 5.
2 - SETTING THE VOLUME
Volume is set using the pipetting pushbutton
(Fig. 1A2) or black adjustment knob (Fig. 1B),
while the value is shown on the digital counter.
The volume shown by the counter is represented
by three digits which should be read from top to
bottom. Typical readings are shown in the fol-
lowing table:
To attain the maximum accuracy, set volume
must be approached from a higher value. If the
desired value is lower than the previous value,
simply use the knob in the pushbutton (Fig. 1A2)
or the black adjustment knob (Fig. 1B) to adjust
the value down to 1/3 turn above the required set-
ting. If the desired value is higher than the pre-
vious value, use the knob in the pushbutton or the
black adjustment ring to adjust the value to 1/3
turn above the required value. The setting should
then be performed in the following manner:
With the volume set to 1/3 above the required
setting, slowly turn the pipetting pushbutton
knob or the black adjustment knob to the
required volume.
Model
Counter
readings
Set
volume
Increments
0
3
5
2
5
0
0
8
5
0
6
5
BPE-10
BPT-10
BPE-50
BPT-50
BPE-200
BPT-200
BPE-300
BPT-300
3.5 µl
6.5 µl
85.0 µl
250.0 µl
0.02 µl
0.10 µl
0.20 µl
1.0 µl
BPM/08/2004 v06 31/07/2004 01:06 PM Page 8