LaMotte AQ-4 Saltwater Aquaculture Kit - Manual

SALT WATER
AQUACULTURE TEST KIT
MODEL AQ-4 · CODE 3635-03
NOTE:It is important to read the instruction
manual before attempting to perform the tests
with the short form instructions provided
below.
*WARNING: Reagents marked with an * are
considered potential health hazards. To view or
print a Material Safety Data Sheet (MSDS) for
these reagents see MSDS CD or
www.lamotte.com. To obtain a printed copy,
contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit
components, use the specified code number.
pH
1. Fill test tube (0106) to 5 mL line with sample
water.
2. Add 8 drops *Wide Range pH Indicator (2218).
Cap and mix.
3. Insert test tube into Octa-Slide Viewer (1100).
Insert pH Octa-Slide Bar (3483). Match color.
4. Record as pH.
AMMONIA NITROGEN
1. Fill test tube (0106) to 5 mL line with sample
water.
2. Add 10 drops *Salicylate Ammonia #1
(3978LWT). Cap and mix.
3. Add 7 drops of *Salicylate Ammonia #2
(3979WT). Cap and mix. Wait 1 minute.
4. Add 7 drops of Salicylate Ammonia #3
(3982WT). Cap and mix wait 20 minutes.
5. Insert test tube into Octa-Slide Viewer (1100).
Insert Ammonia Nitrogen Octa-Slide Bar (3441)
match color.
6. Record as ppm Ammonia Nitrogen (NH
3
-N).
NITRITE NITROGEN
1. Fill test tube (0106) to 2.5 mL line with sample
water.
2. Dilute to 5 mL line with *Mixed Acid Reagent
(V-6278).
3. Use 0.1g spoon (0699) to add 0.1g of *Color
Developing Reagent (V-6281). Cap and mix for
1 minute. Wait 5 minutes.
4. Place test tube into Octa-Slide Viewer (1100)
Insert Nitrite Nitrogen Octa-Slide Bar (3437).
Match color.
5. Record as ppm Nitrite Nitrogen (NO
2
-N).
NITRATE NITROGEN
1. Fill test tube (0106) to 2.5 mL line with sample
water.
2. Dilute to 5 mL line with *Mixed Acid Reagent
(V-6278). Cap and mix. Wait 2 minutes.
3. Use 0.1g spoon (0699) to add one level measure
of *Nitrate Reducing Reagent (V-6279). Cap
and invert 50-60 times in one minute. Wait 10
minutes.
4. Invert sample one time and insert test tube into
Octa-Slide Viewer (1100). Insert Nitrate
Nitrogen Octa-Slide Bar (3413). Match color.
5. Record as ppm Nitrate Nitrogen (NO
3
-N).
ALKALINITY
1. Fill titration tube (0608) to 5 mL line with
sample water.
2. Add 4 drops of *BCG-MR Indicator (2311-EG).
Cap and mix. Sample will turn blue-green.
3. Fill Direct Reading Titrator (0382) with
*Alkalinity Titration Reagent B (4493DR).
4. Titrate sample until blue-green color changes to
pink.
5. Record as ppm Alkalinity (CaCO
3
).
CARBON DIOXIDE
1. Fill titration tube (0608) to 20 mL line with
sample water.
2. Add 2 drops *Phenolphthalein Indicator, 1%
(2246). If sample turns red, no free carbon
dioxide is present. If colorless, proceed to Step 3.
3. Fill Direct Reading Titrator (0380) with *Carbon
Dioxide Reagent B (4253DR).
4. Titrate sample until faint pink color persists for 30
seconds.
5. Record as ppm Carbon Dioxide (CO
2
).
SALINITY
1. Fill titration tube (0608) to 10 mL line with
demineralized water (1151).
2. Fill the 1.0 mL Titrator (0376) to 0 mark with
sample water. Wipe excess water off the Titrator.
3. Dispense 0.5 mL of sample water into titration
tube by depressing plunger until tip is at 0.5
mark. Discard remaining water in Titrator.
4. Add 3 drops of *Salinity Indicator Reagent A
(7460) to titration tube. Cap and gently swirl to
mix. Sample will turn yellow.
5. Fill Titrator (0378) with *Salinity Titration
Reagent B (7461).
6. Titrate sample until color changes from yellow to
pink-brown.
7. Record as ppt Salinity.
DISSOLVED OXYGEN
SAMPLING
1. Rinse sampling bottle (0688-DO). Replace cap.
2. Submerge bottle, then remove cap.
3. Tap sides of bottle to release air bubbles.
4. While bottle is submerged replace cap and
retrieve from water.
5. If air bubbles are present repeat sampling
method.
PRESERVATION
1. Add 8 drops of *Manganous Sulfate Solution
(4167).
2. Add 8 drops of *Alkaline Potassium Iodide
Azide Reagent (7166). Caution. Cap and mix by
inverting several times. Allow precipitate to
settle.
3. Add 8 drops of *Sulfuric Acid, 1:1 (6141WT).
4. Cap and mix until precipitate dissolves. Sample
is now “fixed”.
TEST PROCEDURE
1. Fill titration tube (0608) to 20 mL line with
“fixed” sample. Cap.
2. Fill Direct Reading Titrator (0377) with
*Sodium Thiosulfate, 0.025N (4169). Titrate
sample, swirling between each addition until
color is a very faint yellow.
3. Remove Titrator and cap. Use the pipet (0392)
to add 8 drops of Starch Indicator Solution
(4170PS). Sample should turn blue. Replace cap
and Titrator.
4. Titrate sample until blue color just disappears.
5. Record as ppm Dissolved Oxygen.
LaMOTTE COMPANY
PO Box 329 • Chestertown • Maryland • 21620 • USA
800-344-3100 • 410-778-3100 (Outside U.S.A.) • Fax 410-778-6394 • www.lamotte.com
63635-03-SF • 4.08