LaMotte TL-2 Turf Lab Soil Test Kit - Manual (Page 5)

NI TRATE NI TRO GEN
NOTE: Place Dis penser cap (0693) on *Mixed Acid (V-6278). Save Dis penser
Cap for re agent re fills.
1. Fill test tube (0106) to the 5 mL line with the soil filtrate.
2. Dilute to 10 mL line with *Mixed Acid Reagent (V-6278).
3. Use the 0.1 g spoon (0699) to add 2 level measures of *Nitrate Reducing
Reagent (V-6279). Cap and mix by inverting 50 - 60 times in one minute.
Wait 10 minutes for maximum color development.
NOTE:
At the end of the waiting period, an undissolved portion of the
*Nitrate Reducing Reagent may remain in the bottom of the tube without
affecting the results.
4. Mix the sample one time and insert the tube into the top hole of the
Octa-Slide Viewer (1100). Slide the Nitrate Nitrogen Octa-Slide Bar
(3422) into the Viewer. Match sample color to a color standard. Record as
lb/acre nitrate nitrogen. If the sample color falls between two standards,
take the midpoint between the two standard values as the result.
NOTE:
Values above the highest standard may be determined by diluting
the filtrate and multiplying the results by a dilution factor.
PHOS PHO RUS
1.
Use the 1 mL pipet (0354) to add 1 mL of the soil filtrate to the tube
(0106).
2. Dilute to the 5 mL line with deionized water.
3. Use the 0.5 mL pipet (0353) to add 0.5 mL of *VM Phosphate (4410).
Cap and mix by inverting 3 times. Wait 5 minutes.
4. Use the plain pipet (0352) to add 2 drops of *Reducing Reagent (6405).
Cap and mix. Solution will turn blue.
5. Insert test tube into the Octa-Slide Viewer (1100). Slide the Phosphorus
Octa-Slide Bar (3423) into the Viewer. Match sample color to a color
standard. Record as lb/acre phosphorus. If the sample color falls between
two standards, take the midpoint between the two standard values as the
result.
NOTE:
Values above the highest standard may be determined by diluting
the filtrate and multiplying the results by a dilution factor.
PHOS PHO RUS IN AL KALINE SOILS
A special extraction procedure is used for determining the available phosphorus
content of Western U.S. soils where the pH value is above 7.0.
EX TRAC TION PRO CE DURE
1. Use the 1 mL pipet (0354) to add 1 mL of the *Special NF Extracting
Solution (6362) to the test tube (0701). Dilute to 15 mL line with
deionized water.
2. Use the 1 g spoon (0697) to add 3 measures of soil.
3. Add 0.5 mL of Charcoal Suspension (5638). Cap and mix for 5 minutes.
5.