LaMotte BOD5 TEST KIT BOD User Manual
Page 3
TABLE I: EXPECTED BOD
5
RANGE
Sample added to 300 mL
bottle (mL)
Minimum
(mg/L)
Maximum
(mg/L)
3
210
560
6
105
280
9
70
187
12
53
140
15
42
112
18
35
94
21
30
80
24
26
70
27
24
62
30
21
56
45
14
37
60
11
28
75
8
22
150
4
12
2.
Fill two 300 mL BOD bottles (2-2033) about half full with dilution water.
With an appropriately sized pipet or graduated cylinder dispense the
precalculated amount of the sample into each of the two 300 mL BOD
bottles. Fill each bottle with dilution water and insert stopper. Exclude all air
bubbles.
3.
Fill an additional two 300 mL BOD bottles (2-2033) only with dilution
water. Insert stoppers.
4.
Incubate one bottle containing diluted sample (Step 2) and one bottle
containing only dilution water (Step 3) at 20°C for 5 days. Ensure that the
area above the stopper contains distilled water. Check daily or use bottle
covers to prevent evaporation (not supplied).
5.
Run a dissolved oxygen determination on the remaining BOD bottle from
Step 2. Record the initial dissolved oxygen content as D
1
. Run another
dissolved oxygen determination on the remaining BOD bottle from Step 3.
Record initial dissolved oxygen content for control as C
1
. See Dissolved
Oxygen Determination Procedure.