LaMotte Nitrate-Nitrogen Enzyme Reduction Test Kit - Manual (Page 4)

The Ni trate-Ni tro gen En zyme Re duc tion Kit
The Enzyme Vials contain both freeze-dried NADH and YNaR1. The nitrate
reduction takes place under buffered conditions at the molybdenum site on the
YNaR1 enzyme. The YNaR1 is first reduced by the NADH and then the nitrate
is reduced at the molybdenum site. The nitrite produced is then detected by
running the standard Griess reaction in acidic conditions with sulfanilamide and
N-1 indicator in tablet form to produce a red-violet colored complex that is
proportional to the concentration of nitrate.
Con ver sions
Nitrate test results are expressed as “nitrate-nitrogen” (NO
3
_ N), meaning
“nitrogen that is in the form of nitrate”. Some test kits express levels only as
nitrate (NO
3
). Both expressions refer to the same chemical but express the
concentration in different units of measure. To convert ppm nitrate-nitrogen to
ppm nitrate multiply by 4.4.
ppm Nitrate-nitrogen X 4.4 = ppm Nitrate
LaMotte Com pany
PO Box 329 Chestertown, MD 21620
800-344-3100 • 410-778-3100 • f 410-778-6394
www.lamotte.com
8.07 8137-LP