LaMotte SAB/SAB User Manual

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LaMotte BioPaddles™ TECH DOCUMENT

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Email: [email protected]

For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory.
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LaMotte_BioPaddles_SAB_SAB 9.12

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SAB/SAB

Code 5551


USE:

Selective isolation of fungi (yeasts and molds) (SAB)


Side 1 & 2: Sabouraud Dextrose Agar (SAB) (colorless / slightly hazy)


APPLICATION

Sabouraud Dextrose Agar (SAB) is a modification of dextrose agar described by Sabouraud.

1

SAB is used for cultivating

pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permit
selectivity of fungi.

2

Sabouraud Dextrose Agar is used for determining the microbial content of cosmetics,

3

in the

mycological evaluation of food,

4

and clinically to aid in the diagnosis of yeast and fungal infections.

5

PADDLE AGARS

Sabouraud Dextrose Agar (SAB)

Enzymatic digest of casein and enzymatic digest of animal tissue provide the nitrogen

and vitamin source required for organism growth in SAB. The high concentration of dextrose is included as an energy
source. Agar and a proprietary polymer are the solidifying agents.

CULTURE CONTROLS

10-300 inoculum (CFU)

SAB Agar

Aspergillus niger GROWTH

Candida albicans GROWTH

Escherichia coli

INHIBITED

Microsporum canis GROWTH
Trichophyton mentagrophytes GROWTH

1

Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061.

2

Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl’s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.

3

Curry, A. S., J. G. Graf, and G. N. McEwen, Jr. (eds.). 1993. CTFA Microbiology Guidelines. The Cosmetic, Toiletry, and Fragrance Association, Washington, D.C.

4

U.S. Food and Drug Administration. 1995. Bacteriological analytical manual, 8

th

ed. AOAC International, Gaithersburg, MD.

5

Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). Manual of clinical microbiology, 6th ed. American Society for Microbiology,

Washington, D.C.


BioPaddles™ Colony Identification App

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