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SsoAdvanced™ Universal SYBR® Green Supermix
Manual
Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix User Manual
Ssoadvanced, Universal sybr, Green supermix
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Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix User Manual | 28 pages
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Table of contents
Document Outline
Sso7d Fusion Enzyme Technology
Educational Resources
Reagent Evaluation and Comparison Tutorials
Protocol
Sample Preparation Considerations
RNA Samples
RNA Integrity and Purity
DNA Samples
Plasmid Samples
Assay Design Considerations
Some Key Design Considerations
Procedure
Reaction Mix Preparation and Thermal Cycling Protocol
Real-Time PCR Validation for Gene Expression Experiments
Determining the Optimal Reference Gene
Determining the Dynamic Range of the Reverse Transcription Reaction
Determining the Real-Time PCR Performance Characteristics
Troubleshooting Guide
Ordering Information
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