Bio-Rad Criterion™ Cell User Manual
Page 8
4b. An alternative method is to run the comb down the sides of the cassette to break the weld-
joint and to pull the halves apart.
Fig. 7. Alternative method for opening Criterion Gel cassette after electrophoresis.
5. It is best to remove the gel by floating it off the plate. Invert the gel and plate under fixative
or transfer solution and agitate gently until the gel separates from the plate.
6. Rinse the Criterion tank and lid with distilled, deionized water after use.
Section 3
Maintenance
C
Crriitte
erriio
on
n tta
an
nk
k a
an
nd
d lliid
d
Rinse thoroughly with distilled water after every
use.
Section 4
Troubleshooting Guide
Problem
Cause
Solution
1. "Smile effect" - band pattern
a.
Center of the gel running
a.
Buffer not mixed well or buffer
curves upward at both sides
hotter than either end.
in upper chamber too
of the gel.
concentrated. Remake buffer,
insuring thorough mixing,
especially when diluting 5x or
10x stock.
b.
Power conditions excessive.
b.
Decrease power setting from
200 V to 150 V or fill lower
chamber to the fill line on the
tank.
6