Bio-Rad Silver Stain Plus Kit - Manual (Page 9)

Silver Stain Plus 7
Table A. Times and volumes required to stain 2 gels.
Gel Thickness
0.75 to 1.0 mm
Gel Thickness
1.5 to 3 mm
Step Time Mini
Gel
Large
Gel
Time Mini
Gel
Large
Gel
Fixative 20 min 400 ml 800 ml 30 min 400 ml 800 ml
Water wash 10 min 400 ml 800 ml 20 min 400 ml 800 ml
Water wash 10 min 400 ml 800 ml 20 min 400 ml 800 ml
Stain 20 min 100 ml 300 ml 20 min 100 ml 300 ml
Stop 15 min 400 ml 400 ml 15 min 400 ml 400 ml