Bio-Rad Mini-PROTEAN® Tetra Handcast Systems - Manual (Page 2)

Table of Contents
Page
Section 1 Introduction ..................................................................................................1
Section 2 Pouring Multiple Gradient Gels in the Multi-Casting Chamber...........2
2.1 Calculate the Volume of Acrylamide to Prepare.......................................................2
2.2 Calculate the Chamber Volumes ...............................................................................2
2.3 Reassemble the Multi-Casting Chamber ...................................................................2
2.4 Pour the Gels ..............................................................................................................2
2.4.1
Pour Linear Gradient Gels ............................................................................2
2.4.2 Pour Convex Exponential Gradient Gels......................................................3
Section 3 Pouring One Large-Format Linear Gradient Gel ...................................4
3.1 Calculate the Volume of Acrylamide to Prepare.......................................................4
3.2 Calculate the Chamber Volumes ...............................................................................4
3.3 Set Up the Glass Plate for Casting .............................................................................4
3.4 Pour the Gel................................................................................................................4
3.4.1
Pour a Linear or Concave Gradient Gel from the Top .................................4
3.4.2 Pour a Linear or Convex Gradient Gel from the Bottom.............................5
Section 4 Gradient Former Care and Maintenance .................................................6
Section 5 Product Information ....................................................................................6
Appendix A Estimated Volume of Acrylamide for 12 Mini-PROTEAN 3 Gels .........6