Bio-Rad Mini-PROTEAN 3 Multi-Casting Chamber - Manual (Page 9)

Water
(55 ml)–(36.7 ml + 13.8 ml) = X
X = 4.5 ml
APS
(500 µl) / (100 ml) = ( X µl) / (55 ml)
X = 275 µl
TEMED
10% the APS volume; (275 µl) / 10 = X
X = 27.5 µl
Appendix B
Guidelines for Gel Casting
Gradient Non-gradient Stacker Overlay
Yes – No No Cast monomer solution to the top
of the gel cassettes and insert the
combs immediately.
Yes – Yes Yes Cast monomer solution to the
desired resolving gel height,
overlay and store in buffer once
polymerized. Prior to elec-
trophoresis cast the stacking gel
and insert the combs.
– Yes Yes Yes Cast monomer solution to the
desired resolving gel height,
overlay and store in buffer once
polymerized. Prior to elec-
trophoresis cast the stacking gel
and insert the combs.
– Yes Yes Yes Cast monomer solution to the
desired resolving gel height and
overlay. Once polymerized cast
the stacking gel and insert the
combs. Store in buffer with the
combs inserted.
– Yes No No Cast monomer solution to the top
of the gel cassettes and insert the
combs immediately.
7