Bio-Rad Silver Stains - Manual (Page 2)

Table of Contents
Section 1 Introduction...............................................................1
Section 2 Silver Stain Kit Components ...................................1
Section 3 Important Procedural Information.........................1
3.1 Water Purity .........................................................................1
3.2 Gel Handling .......................................................................2
3.3 Temperature .........................................................................2
3.4 Convenient Stopping Point ..................................................2
3.5 Band Development ..............................................................2
3.6 Destaining............................................................................2
3.7 Storage of Stained Gels .......................................................3
3.8 Photographing Gels .............................................................3
Section 4 Reagent Preparation.................................................4
Section 5 Protocols ....................................................................5
Section 6 Additional Applications............................................7
6.1 Nucleic Acid Staining..........................................................7
6.2 Silver Staining after Coomassie Blue R-250 Staining.........7
6.3 Silver Staining Electrofocusing Slab Gels...........................7
Section 7 Troubleshooting Guide .............................................7
Section 8 Ordering Information...............................................9
Section 9 References................................................................10
9.1 General ..............................................................................10
9.2 Proteins ..............................................................................10
9.3 Nucleic Acids ....................................................................11
9.4 Isoelectric Focusing...........................................................11
LIT34B 6/17/98 2:04 PM Page i