Bio-Rad Total Protein Blot Stains - Manual (Page 4)

1.3 Storage
Stable for at least 6 months when stored at room temperature,
protected from light.
1.4 Materials Required But Not Supplied
a) Staining containers: plastic, preferably polypropylene or
Polyvinyl chloride (PVC) photographic staining trays, such as
Photoquip Cesco-lite 8 x 10 inch, can be used for large-format
gels. Glass dishes are not recommended.
b) UV or blue light transilluminator with Polaroid™ or CCD
camera or a laser-scanning instrument.
1.5 Safety Considerations
No data are available on the toxicity of SYPRO Ruby protein stains.
The dye is comprised of an organic component and a heavy metal
c o m ponent (ruthenium). The SYPRO Ruby IEF stain is an acidic
solution, which may cause burns. Eye protection and gloves should be
worn and general laboratory safety precautions followed while handling
all SYPRO Ruby protein stain products.
1.6 Disposal Considerations
Laws governing the disposal of laboratory chemicals vary by region.
Check local laws for the proper disposal of SYPRO Ruby protein stain
products.
3
1.2 Excitation and Emission
Fluorescence excitation and emission spectra of SYPRO Ruby
protein gel stain.
2
Introduction to SYPRO Ruby protein stains Introduction to SYPRO Ruby protein stains
4006173B.qxd 01/21/2002 7:40 PM Page 2