Bio-Rad CHEF Genomic DNA Plug Kits User Manual
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Table of Contents
Section 1
Introduction......................................................1
1.1
Kit Components ..........................................................2
Section 2
Preparation of Agarose Embedded
Mammalian DNA ............................................3
Section 3
Preparation of Agarose Embedded
Bacterial DNA ..................................................5
Section 4
Preparation of Agarose
Embedded Yeast DNA ....................................8
Section 5
Restriction Enzyme Digestion of Plugs ........11
Section 6
References ......................................................12
Section 7
Additional Reagents for Pulsed Field
Electrophoresis ..............................................13
Section 8
Instruments for Pulsed Field
Electrophoresis ..............................................13
Section 9
Appendix ........................................................14
9.1
Solutions ....................................................................14
9.2
Agarose Concentrations ............................................15
9.3
Hemocytometer Usage ..............................................16
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