Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 6)

4.2 SSCP Gel
Problem Cause Solution
Streaking or DNA Impurities in acrylamide 1. Filter before use. Check shelf
spikes in gel life date of acrylamide solution.
2. Be careful not to pierce wells
while loading samples.
No difference in Incorrect running 1. Insure that the gel contains 7%
band shift conditions. glycerol.
2. Run the gel at 10 °C.
3. Denature samples prior to
loading.
Section 5
References
1. Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K. and Sekiya, T,
Proc. Natl. Acad. Sci. USA, 86, 2766-2770 (1989).
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