Bio-Rad Zeta-Probe Membranes - Manual (Page 2)

Table of Contents
Section 1 Introduction ..........................................................1
Section 2 Nucleic Acid Blotting Protocols ..............................1
2.1 Southern Blotting (DNA Capillary Transfer) .............2
2.2 Northern Blotting (RNA Capillary Transfer) ..............3
2.3 Alkaline Blotting (DNA Capillary Transfer)................4
2.4 Electrophoretic Transfer .........................................5
2.5 DNA Dot Blotting ...................................................8
2.6 RNA Dot/Slot Blotting ............................................9
2.7 DNA Alkaline Fixation ...........................................10
Section 3 Probe Recommendations ....................................10
Section 4 Hybridization Protocols for DNA Probes...............11
4.1 Standard Protocol................................................13
4.2 Formamide Protocol ............................................15
4.3 Alternative Protocol..............................................16
4.4 Oligonucleotide Protocol ......................................17
Section 5 Hybridization Protocols for RNA Probes...............19
Section 6 Probe Stripping and Rehybridization ....................21
Section 7 Troubleshooting ...................................................22
7.1 Nucleic Acids .......................................................22
Section 8 Appendix .............................................................26
Section 9 References ..........................................................28
Section 10 Ordering Information ............................................29
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