Bio-Rad Gel Doc™ XR+ System User Manual

Page 42

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42


Section 6 Troubleshooting

Problem

Possible Cause

Solution

Image is not visible on the
monitor

•

Aperture is closed

•

Incorrect monitor settings

•

Wrong cable connections

•

Open the aperture

•

See your computer manual

•

See Appendix A for correct
cabling

Image is not bright enough

•

Wrong aperture setting

•

Insufficient exposure time

•

Open the aperture

•

Select longer exposure time

Impossible to image whole
sample area

•

Lens is zoomed-in too close

•

Zoom-out the lens

Printout does not look the
same as the image on the
monitor

• Monitor settings are wrong
• Thermal printer settings are

wrong

•

Read the manual for the printer to
ensure proper settings

Light leakage into the
darkroom

•

The lens body is not properly
centered and against the light
seal gasket on the hood

•

Τhe rear hole on the MZL not
closed with the screw

•

Loosen the bracket and move the
camera around, to properly seal
the hood opening or adaptor plate,
as the case may be

•

Close the hole with the black
plastic screw

Hot (very bright) pixels are
seen in the image
(ChemiDoc

™

XRS only)

•

Hot pixels are more
susceptible to dark current
than surrounding pixels.

•

Select reference by clicking on
the appropriate box in the Options
window

Hot (very bright) pixels are
seen in the image
(Gel Doc XR only)

•

Corrupted reference dark
image

•

Click on Options button and then
click on Reset Reference Dark

ChemiDoc XRS camera will
not respond/camera not
found

•

Camera power may be turned
off

•

Controller cables may not be
seated properly

•

Software driver for the
camera is missing

•

Computer power-saving
modes may be interfering
with the camera driver

•

Multiple FireWire devices
are installed on the same
computer

•

Camera may be defective

•

Turn ON the power to the camera

•

Make sure that all cables are
connected properly

•

Install Quantity One software
again if the camera driver is not
present

•

Disable power-saving modes on
the computer

•

Exit out of the Quantity One

software and turn off power to the
ChemiDoc XRS camera

•

Power the camera back on and
restart the software

•

Replace camera

ChemiDoc XRS image will
not autoexpose

•

The sample is too bright and
saturates the image at the
minimum possible exposure

•

Close the Iris control until
autoexpose is possible

Unable to focus on the
sample using white light
transilluminator or
conversion screen

•

Aperture is open too wide
causing a shallow depth of
field

•

Close the iris slightly and then
focus again. Repeat until good
focus is achieved

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