Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 2)

Table of Contents
Introduction 1
Principle 2
Kit Contents and Storage 4
Recommended Materials 5
Assay Workflow 6
Important Considerations 7
Detailed Instructions 7
1. Plan Plate Layout 8
2. Prepare Instrument 9
3. Prepare Wash Method 10
4. Prepare Standards and Controls 11
5. Prepare Samples 13
6. Prepare Coupled Beads 16
7. Run Assay 17
8. Read Plate 22
Troubleshooting Guide 29
Plate Layout Template 34
Calculation Worksheet 35
Safety Considerations 37
Legal Notices 37
Ordering Information 38