Bio-Rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays - Manual (Page 4)

11. Wash the plate three times with 100 µl wash buffer.
12. Vortex the diluted (1x) SA-PE. Add 50 µl to each well.
13. Repeat Step 5. See table for incubation time.
14. Wash the plate three times with 100 µl wash buffer.
15. Resuspend beads in 125 µl assay buffer. Cover plate as in Step 5 and
shake the plate at 850 ± 50 rpm for 30 sec.
16. Remove sealing tape and read the plate using the settings below.
Bio-Plex Pro Assay Quick Guide 4
Life Science
Group
Sig 1212 10024985 Rev A
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800
Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699
Canada 905 364 3435 China 86 21 6169 8500
Czech Republic 420 241 430 532 Denmark 44 52 10 00
Finland 09 804 22 00 France 01 47 95 69 65 Germany 089 31 884 0
Greece 30 210 9532 220 Hong Kong 852 2789 3300
Hungary 36 1 459 6100 India 91 124 4029300 Israel 03 963 6050
Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460
Mexico 52 555 488 7670 The Netherlands 0318 540666
New Zealand 64 9 415 2280 Norway 23 38 41 30
Poland 48 22 331 99 99 Portugal 351 21 472 7700
Russia 7 495 721 14 04 Singapore 65 6415 3188
South Africa 27 861 246 723 Spain 34 91 590 5200
Sweden 08 555 12700 Switzerland 026 674 55 05
Taiwan 886 2 2578 7189 Thailand 800 88 22 88
United Kingdom 020 8328 2000
Settings for optimal sensitivity on the Bio-Plex 100, 200, 3D or similar system.
Assay
Low PMT, RP1 High PMT, RP1
Bio-Plex Pro human cytokine (group I and II) • —
Bio-Plex Pro mouse cytokine (group I, II, and III) • —
Bio-Plex Pro rat cytokine (group I) — •
Note: Use default instrument settings for Bio-Plex ® MAGPIX ™ .
The Bio-Plex suspension array system includes
fluorescently labeled microspheres and instrumentation
licensed to Bio-Rad Laboratories, Inc. by the Luminex
Corporation.