Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual

This guide can be used to prepare and run a full 1 x 96-well assay plate. For
more information on a given step, refer to the complete instruction manual.
New users can download the manual at www.bio-rad.com/bio-plex.
Initial Preparation
1. Plan the plate layout.
2. Start up/warm up the Bio-Plex ® system (30 min).
n
Bring assay buffer, wash buffer, and sample diluent to room
temperature (RT). Keep other items on ice until needed
n
Begin to thaw frozen samples
3. Prime wash station for flat bottom plate or set vacuum manifold to
–1 to –3" Hg for filter plate.
4. Follow the prompts in Bio-Plex Manager ™ software to calibrate the
system. This can be done now or during an incubation step.
5. Mix 1 volume of Bio-Plex standard diluent with 3 volumes of Bio-Plex
sample diluent (each supplied in the kit). The resulting solution is used
for reconstitution and subsequent dilution of standards. This results in a
serum-matrix based diluent that mimics the matrix in 1:16 diluted serum
and plasma samples. For samples in serum-free media and other biological
fluids, use a diluent that most closely matches the sample matrix. Add carrier
protein such as BSA at a final concentration of 0.5% (w/v).
Bio-Plex Pro ™ Assays
Quick Guide 5
Important: Pay close attention to vortexing, shaking, and incubation instructions.
Deviation from the protocol may result in low assay signal and assay variability.
For use with
Instruction Manual #
TGF-b Assays 10024984