Bio-Rad Affi-Gel Protein A Media - Manual (Page 3)

Section 1
Econo-Pac Protein A Kit
1.1 Introduction
The Econo-Pac protein A kit is based upon Bio-Rad’s Affi-Gel ®
protein A MAPS ® II methods and contins everything necessary to
purify monoclonal antibodies from ascites or serum. Affi-Gel protein
A agarose consists of purified protein A coupled to crosslinked
agarose beads via chemically stable amide bonds. The Econo-Pac pro-
tein A column contains 2 ml of Affi-Gel protein A with a total capac-
ity of 10-14 mg mouse IgG
1
when used with the MAPS II buffers
(included).
The Econo-Pac protein A kit provides a simple and convenient
method for the purification of all IgG subclasses, including IgG
1
,
from ascites fluid. Sample preparation has been simplified by the
use of the Econo-Pac 10DG desalting columns. Binding, elution,
and regenerations steps can be completed with only one column vol-
ume of buffer per step, making the total procedure fast and easy.
1.2 Kit Components
Econo-Pac protein A column One Econo-Pac column
packed with 2 ml of Affi-Gel
protein A agarose.
Econo-Pac 10DG desalting
columns
Four Econo-Pac 10DG
desalting columns.
Binding buffer One bottle (471 g) of buffer
solids, reconstitution volume
= 1,500 ml.
Elution buffer One bottle (25 g) of buffer
solids, reconstitution volume
= 1,000 ml.
Regeneration buffer One bottle containing 400
ml. CAUTION: Contains
methanol. DO NOT MOUTH
PIPETTE.
Instruction manual
The Econo-Pac protein A kit contains a sufficient quantity of
reagents to purify approximately 300 mg of mouse IgG
1
.
1
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