Bio-Rad Bio-Scale™ Mini Affi-Prep® Protein A Cartridges - Manual (Page 5)

loading capacities Rat, sheep, bovine, equine,
goat, rabbit, dog, and porcine
IgG = 9-16 mg/ml
IgM = 5-7 mg/ml*
Flow rate 2,000 cm/hr maximum; 150
cm/hr recommended. We
recommend the use of the
Bio-Rex
® MP
chromatography columns for
medium pressure Affi-Prep
protein A applications.
Pressure limit 1,000 psi
Chemical stability pH 2-14, 1 N NaOH, 8 M
urea, 50% methanol, 6 M
guanidine-HC1
Shipping buffer 0.05 M sodium phosphate,
pH 7.5, containing 0.05%
sodium azide
Shelf life 1 year at 4 °C
*Approximately 50% of all IgM’s will bind to protein A.
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stringent column wash. This NaOH wash should
only be used after the regular methanol regeneration
step. For complete sanitation (i.e. removal of
endotoxins and DNA) the support can be washed
with 1.0 N NaOH. This is an acceptable method of
sanitation for FDA purposes.
7. If the column will be re-used right away, re-
equilibrate the column with at least 5 column
volumes of MAPS binding buffer. If the column is to
be stored, equilibrate the column with a mild neutral
buffer such as 0.05 M sodium phosphate, pH 7.5,
containing 0.02-0.05% sodium azide.
Section 2
Production Information
Monoclonal antibody Mouse IgG
1
= 8-10 mg/ml
loading capacities IgG
2a
= 13-15 mg/ml
IgG
2b
= 13-15 mg/ml
IgG
3
= 8-10 mg/ml
Polyclonal antibody Human IgG = 16-23 mg/ml
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LIT230B 9/2/98 10:28 AM Page 4