Bio-Rad CM Affi-Gel Blue Gel - Manual (Page 2)

Introduction
CM Affi-Gel blue gel is a bifunctional affinity/ion exchange
chromatography matrix prepared by coupling Cibacron blue
F3GA and carboxymethyl (CM) groups to Bio-Gel
® A-5m
crosslinked agarose gel. The Cibacron blue functions as an ionic,
hydrophobic, or sterically active binding site for proteins with
dinucleotide folds, such as albumin.
The carboxymethyl functional group functions as a cation
exchanger and will bind proteins with isoelectric points lower
than the pH of the mobile phase. (At pHs below 4.5 the car-
boxymethyl group will be protonated and will not function as an
ion exchanger.) This bifunctionality allows CM Affi-Gel blue
gel to bind both albumin and protease from serum. Dialysis of
o NH 2
o
NH
SO 2 ONa
NH
N
N
N
O
Crosslinked
Agarose
NH
R 1
R 2
R 1 = H or SO 2 ONa
R 2 = SO 2 ONa or H
1
Cibacron blue coupled to agarose.
LIT308C 9/3/98 7:13 AM Page 1