Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges - Manual (Page 6)

2
Section 2
Technical Description
Composition Highly Crosslinked Polymer
Particle Size Range 53–61 µm
Ligand Recombinant Protein A
Coupling Chemistry Epoxy
Dynamic Binding Capacity 1 150 cm/hr 30 ± 3 mg/ml
300 cm/hr 25 ± 2 mg/ml
450 cm/hr 20 ± 2 mg/ml
Chemical Stability 2 10 mM Hydrochloric acid
6 M Guanidine hydrochloride
0.1 M Arginine (pH 2.8)
0.1 M Citrate (pH 2.8)
0.1 M Glycine (pH 2.8)
Working pH Range 3–11
Cleaning-In-Place (CIP) 6 M Guanidine hydrochloride
10 mM Hydrochloric acid
0.1 M Sodium hydroxide
1 M Acetic acid/20% Ethanol
Recommended Mobile Phase Velocity Range 100–600 cm/hr
Working Temperature Range 2–40ºC
Delivery Conditions 50% slurry in 20% Ethanol
Storage Conditions 2–8ºC
1 Minimum 20 mg/ml at 300 cm/hr; 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human
IgG in 1.1 x 10 cm column
2 No significant change in chromatographic performance after 24 hr storage at room temperature.
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