Bio-Rad AG® MP-50 Cation Exchange Resins User Manual

Page 11

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Section 8
Storage

The resins are stable for at least 2 years when stored

in the original, unopened container at room temperature
and protected from ultraviolet light.

Section 9
Stability

The resins are stable in acid, base, and organic

solvents, and may be autoclaved. To prevent bacterial
growth during prolonged storage of a poured column,
use a preservative such as 0.05% sodium azide or
thimerosol or 20% organic solvent such as methanol or
ethanol.

Table 7. Cation Exchangers for Sample
Preparation

Application

Resin

Reference

Cation removal from

AG 50W-X8 Ochiai, M.,

J. Chromatog., 194,

monosaccharides

resin

224 (1980).

Removal of cations

AG 50W-X8 Hoffer, E. M., Kothny, E. L. and

from sulfate

resin

Appel, B. R., Atmospheric
Environment,
13, 303 (1979).

17

6.5 Notes

The experimental error is only that inherent in the

titration procedure. The error due to the ion exchange
itself is less than that of the titration. To avoid the error
due to interfering carbonate ions, neutralize alkaline tap
water with 0.1 M HCl, one drop at at time, to the methyl
orange end point.

The column may be used several times before

regeneration is necessary. To regenerate the resin, wash
it by passing approximately 50 ml of 3 M HCl through
the column, followed by 75 ml of distilled water.

Section 7
Applications

Strong cation exchange resins are used for sample

preparation, metal separations, weak acid separations,
peptide separations, amino acid separations, and nucleotide
separations. Tables 7-10 summarize the applications.

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LIT203B 6/17/98 12:00 PM Page 16

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