Bio-Rad Chelex® 100 Resin User Manual
Page 11
![background image](/manuals/600445/11/background.png)
Application
Reference
Removal of metals from cell
Bosron, W. F., Kennedy
suspension
F. S. and Vallee, B. L.,
Biochem., 14, 2275 (1975).
Batch removal of metals from
Agarwal, M., Bennett,
urine
R. B., Stump, I. G. and D’Avria,
J. M., Anal. Chem., 47, 924
(1975).
Removal of metals from enzyme
Dunn, M. F., Pattison,
solutions
S. E., Storm, M. C. and Quiel,
E., Biochem., 19, 718 (1980).
Removal of metals from guinea
Amiraian, K., McKinney,
pig complement
J. A. and Duchna, L.,
Immunology, 26, 1135 (1974).
Removal of aldehydes and
Ray, W. J. and
peroxides from polyethylene
Pavathingal, J. M., Anal.
glycol
Biochem., 146, 307 (1985).
Removal of interfering
Brandt, S. J., Dougherty,
components from myo-inositol
R. W., Lapetina, E . G. and
Niedel, J. E., Proc. Nat. Acad.
Sci., 82, 3277 (1985).
Calcium removal from protein
Putnam-Evans, C. L.,
kinases
Harmon, A. C. and Cormier,
M. J., Biochem., 29, 2488 (1990).
Removal of divalent cations
Devlin, C. C. and Grisham,
from NMR stock solutions
C. M., Biochem., 29, 6192
(1990). Chuknyisky, P. P.,
Rifkind, J. M., Tarien, E., Beal,
R. B. and Eichhorn, G. L.,
Biochem., 29, 5987 (1990).
19
Table 2. Sample Applications with
Chelex 100 Resin
Application
Reference
Preparation of metal-free apo-
O’Keefe, E. T., Hill, R. L. and
enzyme
Bell, J. E., Biochem., 19, 4954
(1980).
Removal of calcium from
Chiesi, M. and Inesi, G.,
sarcoplasmic reticulum vesicles
Biochem. 19, 2912 (1980).
Removal of extraneously bound
Barker, R., et al., Biochem.
metal ions from enzymes prior
J., 177, 289 (1979).
to NMR and ESR studies
Batch removal of calcium from
Raymond, F A. and
whole blood
Weinshilboum, R. M., Clin.
Chim. Acta, 58, 185 (1975).
Removal of metals from ATP
Sontheimer, G. M., et al.,
Biochem., 26, 2701 (1987).
Removal of metals from buffer,
Knapp, G., et al., J. Anal.
brine, and biological solutions
Atomic Spectrometry, 2, 611
(1987); Laue, T. M., et al.,
Biochem., 28, 4762 (1989).
Removal of metals from S100b
Baudier, J., et al.,
and melittin
Biochem., 26, 2886 (1987).
Purification of dinucleotides
Reinhardt, C. G. and Krugh,
T. R., Biochem., 17, 4845
(1978).
18
LIT200B 6/17/98 11:50 AM Page 18