Bio-Rad Chelex® 100 Resin User Manual

Page 11

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Application

Reference

Removal of metals from cell

Bosron, W. F., Kennedy

suspension

F. S. and Vallee, B. L.,

Biochem., 14, 2275 (1975).

Batch removal of metals from

Agarwal, M., Bennett,

urine

R. B., Stump, I. G. and D’Avria,
J. M., Anal. Chem., 47, 924
(1975).

Removal of metals from enzyme

Dunn, M. F., Pattison,

solutions

S. E., Storm, M. C. and Quiel,
E., Biochem., 19, 718 (1980).

Removal of metals from guinea

Amiraian, K., McKinney,

pig complement

J. A. and Duchna, L.,
Immunology, 26, 1135 (1974).

Removal of aldehydes and

Ray, W. J. and

peroxides from polyethylene

Pavathingal, J. M., Anal.

glycol

Biochem., 146, 307 (1985).

Removal of interfering

Brandt, S. J., Dougherty,

components from myo-inositol

R. W., Lapetina, E . G. and
Niedel, J. E., Proc. Nat. Acad.
Sci.
, 82, 3277 (1985).

Calcium removal from protein

Putnam-Evans, C. L.,

kinases

Harmon, A. C. and Cormier,
M. J., Biochem., 29, 2488 (1990).

Removal of divalent cations

Devlin, C. C. and Grisham,

from NMR stock solutions

C. M., Biochem., 29, 6192
(1990). Chuknyisky, P. P.,
Rifkind, J. M., Tarien, E., Beal,
R. B. and Eichhorn, G. L.,
Biochem., 29, 5987 (1990).

19

Table 2. Sample Applications with
Chelex 100 Resin

Application

Reference

Preparation of metal-free apo-

O’Keefe, E. T., Hill, R. L. and

enzyme

Bell, J. E., Biochem., 19, 4954
(1980).

Removal of calcium from

Chiesi, M. and Inesi, G.,

sarcoplasmic reticulum vesicles

Biochem. 19, 2912 (1980).

Removal of extraneously bound

Barker, R., et al., Biochem.

metal ions from enzymes prior

J., 177, 289 (1979).

to NMR and ESR studies

Batch removal of calcium from

Raymond, F A. and

whole blood

Weinshilboum, R. M., Clin.
Chim. Acta
, 58, 185 (1975).

Removal of metals from ATP

Sontheimer, G. M., et al.,
Biochem., 26, 2701 (1987).

Removal of metals from buffer,

Knapp, G., et al., J. Anal.

brine, and biological solutions

Atomic Spectrometry, 2, 611
(1987); Laue, T. M., et al.,
Biochem., 28, 4762 (1989).

Removal of metals from S100b

Baudier, J., et al.,

and melittin

Biochem., 26, 2886 (1987).

Purification of dinucleotides

Reinhardt, C. G. and Krugh,
T. R., Biochem., 17, 4845
(1978).

18

LIT200B 6/17/98 11:50 AM Page 18

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