Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 11)

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Table 3. Products for buffer exchange.
Sample
Volume
Product MW Cutoff Catalog
50–100 µl Bio-Spin ® columns 6 kD 732-6002
Bio-Spin 30 columns 30 kD 732-6006
0.1–3 ml Bio-Scale ™ mini
cartriges
6 kD 732-5304
Up to 3 ml Econo-Pac ® 10DG6
columns
6 kD 732-2010
Unlimited Bio-Gel ® P-6DG gel 6 kD 150-0738
3.2 General Purification Protocol
Recommendations on general purification protocols
may be found for each chromatography media in its
corresponding instruction manual. Please refer to
Table 2 for literature number, and download at
www.bio-rad.com/process . For best results and
increased column life, samples and buffers should be
degassed and filtered through a 0.45 µm filter.
3.3 Purification Scale-Up
For quick scale-up, two or three columns of the same
type can be connected in series. Backpressure will
increase with columns in series, so care should be
taken to maintain pressures ≤ 20 bar for 1ml and
≤ 30 bar for 5 ml. Foresight columns are available in
1 and 5 ml column formats. All chromatography
media are designed for process scale and are
available in larger bulk quantities for uses from
preclinical to commercial. All media are fully
supported with regulatory support files. In addition,
Bio-Rad carries an extensive line of empty
chromatography columns for use in laboratory and
process scale applications.