Hudson Robotics SoftLinx 5.0 User Manual

Page 35

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All steps can be dropped from the toolbox to the canvas to create the pattern above. Protocols run from
start to finish, top to bottom.

3. Test the protocol.

This should complete the goal for a single plate. It is recommended to run this protocol now, to ensure that
it acts the way you want it to run, and that all instruments are running properly.

Ensure that the correct plate types and initial plate locations are defined for this protocol. A 96 well plate,
with initial plate location at stack 1, is recommended.

However, we are not done. This protocol is supposed to run 20 times. To repeat parts of a protocol, use a
loop step, and place the items you wish to repeat within the loop.


There are multiple ways to set up this loop. Users can either set the loop up to run forever and empty stack
1, or run the loop a specific amount of times, or use variables to determine how many times a loop should
run. In the example below, we can use the instrument variable "Crane.StackCount(1) > 0" to tell the loop to
run until the number of plates in the Crane's stack 1, as detected by the Crane, is 0.

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