ChemoMetec SCC-400 - Manual (Page 34)

6!NucleoView!SCC0400!!
20!
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Figure,24,.,Instrument,dialogue,
High,Precision,Mode!
When!determining!the!SCC!of!a!sample!the!instrument!takes!a!number!of!measurements!
by!placing!a!portion!of!the!sample!in!the!sample!compartment!and!recording!its!image!for!
counting!of!cells.!The!number!of!such!sub0measurements!relates!directly!to!the!volume!of!
milk!that!has!been!analysed.!By!default,!e.g.!Normal%Precision%Mode,!the!instrument!
analysis!about!18µL!of!sample!mixture,!corresponding!to!about!6µL!of!milk!being!analysed.!
By!setting!the!instrument!in!High%Precision%Mode!twice!the!normal!volume!of!sample!
mixture!is!analysed!when!the!SCC!is!below!approximately!500,000!cells/mL.!Therefore!in!
High%Precision%Mode!a!total!of!12µL!of!milk!is!analysed.!!
Priming,Time!
The!pump!of!the!instrument!is!a!peristaltic!pump!and!its!pump!volume!can!change!with!
time.!To!make!it!possible!to!compensate!for!this!the!Priming%Time!of!the!pump!can!be!
adjusted!by!entering!the!time.!The!time!should!be!long!enough!so!that!ALL!the!sample!
mixture!is!emptied!from!the!sample!container!during!measurement,!while!it!should!not!be!
so!long!that!air!is!present!in!the!sample!compartment!during!analysis.!!
Sample,Compartment!
The!Sample!Compartment!of!the!instrument!defines!the!thickness!of!the!sample!liquid!that!
is!analysed.!This!thickness!is!accurately!determined!during!production.!It!is!important!that!
this!information!is!entered!correctly!in!order!to!maintain!the!accuracy!of!the!
NucleoCounter!SCC0400.!
Save,Collected,Images!
In!normal!operation!the!NucleoView!software!only!saves!summary!data!from!each!
measurement.!During!testing!and!trouble0shooting!it!is!advised!to!set!the!"Save!Collected!
Images"!status!to!on,!which!means!that!for!the!active!session!all!sample!information!will!
be!saved!to!disk.!Note!that!this!feature!is!automatically!turned!off!during!start0up.!!
6.2.5 Tools-L-Barcode-
A!barcode!reader!can!be!used!to!enter!sample!identification,!as!well!as!initiating!the!
measurement.!This!is!done!by!activating!the!Tools%U%Barcode!menu!item.!