Sonics VCX750 - Manual (Page 31)

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Fractional protein release, Rp, is calculated using the following equation and
multiplying the result by 100:
Rp = Cf – Cb
Ct – Cb
Cf = Free protein
Ct = total protein
Cb = Background protein
This gives the actual disruption percentage, taking into account the background levels
of protein before disruption.