Sonics VC750 (Serial No. "Y through "AB")" User Manual

Page 29

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26

Fractional protein release, Rp, is calculated using the following equation and
multiplying the result by 100:

Rp = Cf – Cb

Ct – Cb

Cf = Free protein
Ct = total protein
Cb = Background protein

This gives the actual disruption percentage, taking into account the background levels
of protein before disruption.

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