Physitemp Instruments BFS-MP 6310 Rev1 Pak400 2 User Manual

Page 6

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4.7

REMOVING SPECIMEN FROM STAGE

Press the Freeze/Thaw switch and hold in the QUICK THAW position for a few seconds. Allow the
stage to warm up until specimen slides off easily. Wipe stage dry.

4.8

TYPICAL STAGE PERFORMANCE

The degree of cold obtained is dependent on the temperature of the cooling water. The stage temperature
should reach 55-65° below that of the cooling water. Therefore, a stage temperature of -35°C can be
achieved under normal conditions. 1/2” thickness of liver which covers the stage should freeze within
15-20 minutes. Temperature throughout the tissue should drop to -20°C in a further ten minutes. Tissue
under 1/16” in thickness should be ready for sectioning within 90 seconds after switching on.

4.9

MEASUREMENT AND CONTROL OF TISSUE TEMPERATURE

One of the main advantages of these freezing stages is the ease with which the temperature of frozen tis-
sue can be changed. Optimum cutting temperature varies for different tissue and is best determined by
experiment. the following temperatures have been found satisfactory:

TISSUE

TEMPERATURE °C

Kidney -15

Brain

-18

Liver

-18

Thyroid

-20

Skin

-30

Keloid

-35

Patella

-40

4.10

ADJUSTING THE VIEWING ANGLE OF THE INSTRUMENT

The two front feet can be flipped down to adjust the viewing angle. Rotate the feet down and towards
the front until they lock in position. To return the original position, pull down slightly then rotate back..

5.0

TROUBLESHOOTING

1 Controller display fails to illuminate when power is turned on

Check power cord continuity from the AC outlet to the input socket on the rear of the controller. Check
the fuse next to the AC input socket in the rear panel. If these are both OK, try a different AC outlet

2 Fault Condition lamp is illuminated

As a safety feature, this unit has power supply short circuit protection. In the event that the output
becomes short circuited by a wiring fault, a protection circuit built in the power supply senses the
increase in current and shuts off the output power. Before output power can be restored to the controller,
the AC power supply must be turned off and the fault condition corrected. Consult our engineering
department for further details.

If the PTU-3 or ECV-1 is being used, check for adequate water flow as described in section 3.1. Touch
the silver heat sink, the middle segment of the stage, to see if is is hot to the touch -- this would indicate
a low or inadequate flow rate.

If water flow is OK, the stage or controller may be defective. In order to check the stage, turn off the
power to the controller and remove the stage connection from the rear of the controller by turning the

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QX CW shared\Operating Manuals\EB BFS-MP Pak400 Rev1 1010

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