Hoefer SE900 User Manual
Page 35
• p29
Laemmli Gel (per 200 ml each gel solution)
Gradient Gels Solution
200 ml LIGHT
5.0%
7.5% 10.0% 12.5% 15.0% 17.5%
Acrylamide Stock (Soln. #1) 33.3
50.0
66.7
83.3 100.0 116.7
1.5 M TrisCl,
50.0
50.0
50.0
50.0
50.0
50.0
pH 8.8 (Soln. #2)
10% SDS (Soln. #3)
2.0
2.0
2.0
2.0
2.0
2.0
Deionized H
2
O
112.2
95.5
79.0
62.4
45.8
29.1
10% APS (Soln. #4)
2.0
2.0
2.0
2.0
2.0
2.0
10% TEMED (Soln. #5)
0.50
0.46
0.34
0.27
0.23
0.20
Final Volume
200.0
200.0
200.0
200.0 200.0 200.0
200 ml HEAVY
7.5% 10.0% 12.5% 15.0% 17.5% 20%
Acrylamide Stock (Soln. #1) 50.0
66.7
83.3
100.0 116.7 133.3
1.5 M TrisCl,
50.0
50.0
50.0
50.0
50.0
50.0
pH 8.8 (Soln. #2)
10% SDS (Soln. #3)
2.0
2.0
2.0
2.0
2.0
2.0
Deionized H
2
O
83.0
66.5
49.9
33.3
16.6
0.0
10% APS (Soln. #4)
2.0
2.0
2.0
2.0
2.0
2.0
10% TEMED (Soln. #5)
0.46
0.34
0.27
0.23
0.20
0.17
Glycerol
12.50
12.50
12.50
12.50 12.50 12.50
Final Volume
200.0
200.0
200.0
200.0 200.0 200.0