Hoefer SG500 - Manual (Page 10)

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4
Close the slide valve and add a portion of the final
solution to the reservoir (back) chamber.
5
Carefully open the slide valve and allow just enough
solution to flow through the connector channel to fill
it to the edge of the mixing chamber, then close the
valve. Be sure no large bubbles remain to obstruct
flow through the channel.
6
Add the required volume of the starting solution to the
mixing chamber. With the stopper tubing open, insert
the stopper assembly securely into the mixing cham-
ber (Fig 4). Clamp off the tubing on the vent airtight.
7
Start the magnetic stirrer and unclamp the outlet
tubing if needed.
8
Simultaneously open the slide valve and start
the pump.
9
Stop the pump and remove the tubing from the
receiving container as soon as the last of the solution
has left the reservoir chamber.
Disconnect tubing. Flush and rinse all parts
thoroughly with distilled water after use.
Fig 4. Gradient maker with
plunger in place.
Note: If the gradient volume
is larger than the reservoir will
hold, the additional volume
can be added during the gradi-
ent delivery. Do not allow the
reservoir chamber to go dry
as bubbles will be pulled into
the mixing chamber, changing
the dilution volume and
gradient shape.
Note: The entire solution
remaining in the mixing cham-
ber is at the final concentration
and does not contribute further
to the gradient. If desired, it
can be used as a displacement
solution for convex gradients
unless it contains acrylamide.