Hoefer SUB Series - Manual (Page 29)

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SUB15, SUB20, SUB20C, SUB25,
SUB25C and SUBHT
Units with removable gel casting trays and
gel casting gates.
1
Place the casting gates into the slots provided in
the gel casting tray with the end silicone rubber
gaskets facing outwards.
2 Place the gel unit on a level surface.
3 Position the required combs into the slots
provided in the gel casting tray.
4 Prepare 115, 200 or 375 mls of the desired
% agarose for units SUB15/SUBHT, SUB20/
SUB20C, or SUB25/SUB25C respectively. This
will give a gel depth of 5 mm. For a 10 mm deep
gel, prepare 230, 400 or 750 mls respectively.
5 Using a 1 ml disposable pipette, seal the inside
of the casting gates with some of the cooled
agarose.
6 Pour in the agarose smoothly so as not to gener-
ate bubbles. If bubbles do occur, these can be
smoothed to the side of the gel and dispersed
using a clean gloved hand. Allow the agarose to
set, ensuring that the gel remains undisturbed.
7 Carefully remove the gel casting gates and
comb(s).
8 Place the gel casting tray in the running position
such that the wells are nearest to the black elec-
trode (cathode).
9 Fill the tank with running buffer so that the
buffer just submerges the gel.
IMPORTANT! Ensure that the
agarose has cooled to between
50 and 60 °C to prevent
apparatus distortion.