SKC 225-9611 BioStage Single-stage Bioaerosol Impactor - Manual (Page 4)

4
Sampling
Caution: Sanitize hands or wear disposable gloves. Sanitize hands and clean
the BioStage Impactor in between samples.
Caution: Never use agar that has expired, displays visible cracks, or has been
contaminated.
Caution: Allow agar plates to warm up to ambient temperature
(approximately 20 minutes) before use.
Caution: When sampling indoors, close all doors and windows that could
affect airfl ow in the sampling area.
1. Calibrate the fl ow rate (see Calibration with a QuickTake 30 Sample Pump).
2. Remove the calibration adapter, tubing,
and fl owmeter. The BioStage outlet should
remain connected to the pump inlet with
fl exible tubing. If using the Mounting
Bracket accessory (Cat. No. 228-9531), see
Operating Instructions 40066 supplied with
the accessory.
3. Remove the inlet cone and jet classifi cation
stage from the BioStage. Place a new,
unexposed agar plate into the base plate
(see Assembly). Remove the lid from the agar
plate and place in a clean resealable bag.
Reassemble the impactor.
4. Turn on the vacuum pump and sample for 2 to 5 minutes.
Caution: Sampling too long can cause overgrowth of sample. Sampling times
that are too short can cause false negatives.
5. After sampling is complete, turn off the pump and disconnect the fl exible
tubing from the BioStage outlet.
6. Remove the agar plate (see Removing the Agar Plate), sterilize the sampler (see
Cleaning and Sterilizing), and insert a fresh agar plate. Reassemble the sampler
and take additional samples (see Note below). Repeat sampling as needed.
Note: An additional indoor control sample should be taken in a non-complaint
area. Outdoor samples should be collected for comparison to indoor samples.
Clearly mark each sample. Sterilize the sampler between each sample period
(see Cleaning and Sterilizing).
BioStage mounted on
QuickTake 30 Pump with
mounting bracket accessory